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The museum was founded by U.S. Army Surgeon General William A. Hammond as the Army Medical Museum (AMM) in 1862; it became the NMHM in 1989 and relocated to its present site at the Army's Forest Glen Annex in 2011.
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The AMM was established during the American Civil War as a center for the collection of specimens for research in military medicine and surgery. In 1862, Hammond directed medical officers in the field to collect "specimens of morbid anatomy...together with projectiles and foreign bodies removed" and to forward them to the newly founded museum for study. The AMM's first curator, John H. Brinton, visited mid-Atlantic battlefields and solicited contributions from doctors throughout the Union Army. During and after the war, AMM staff took pictures of wounded soldiers showing the effects of gunshot wounds as well as results of amputations and other surgical procedures. The information collected was compiled into six volumes of The Medical and Surgical History of the War of the Rebellion, published between 1870 and 1883.

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